Sarvtin Mehdi Taheri; Hedayati Mohammad Taghi; Abastabar Mahdi; Shokohi Tahereh
Volume 17, Issue 4 , 2014, , Pages 134-137
Abstract
Background: Psoriasis is an immune mediated skin disorderwhich is mainly characterized by abnormal proliferation anddifferentiation of keratinocytes. It is believed that Debaryomyceshansenii (Candida famata) can colonize skin and mucous membranesof psoriatic patients and exacerbate psoriatic lesions ...
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Background: Psoriasis is an immune mediated skin disorderwhich is mainly characterized by abnormal proliferation anddifferentiation of keratinocytes. It is believed that Debaryomyceshansenii (Candida famata) can colonize skin and mucous membranesof psoriatic patients and exacerbate psoriatic lesions via toxins,antigens, and proteins. The aims of this study were to evaluateDebaryomyces hansenii colonization and its protein profile inpsoriatic patients.Method: Fifty-one patients with psoriasis vulgaris and 51 healthyindividuals were enrolled in the study. Skin and oral specimensfrom all participants were cultured on the CHROMagar Candidamedium. Isolated yeast like fungi were identified using thesequence of the D1/D2 domain of the 26S rRNA gene. Debaryomyceshansenii proteins were analyzed using Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis.Result: Debaryomyces hansenii was only isolated from the oralcavity of 7.84% of the patients. The extracts obtained from variousDebaryomyces hansenii isolates had 45 protein bands, which rangedfrom 18 to >180 kDa. Secretory proteins were seen only in twoisolates, which ranged from 35 to100 kDa.Conclusion: Debaryomyces hansenii can colonize oral cavity ofpatients with psoriasis. In addition, various Debaryomyces hanseniiisolates have different somatic proteins, which may have a rolein provocation and exacerbation of psoriasis.
Mirhendi Seyed Hossein; Hedayati Mohammad Taghi; Omidi Khoshghadam; Zand Niloofar Jalali; Didehdar Mojtaba; Afshar Parvaneh
Volume 10, Issue 3 , 2007, , Pages 73-228
Abstract
Background and aim: Dermatophytosis (tinea, ringworm) is the infection of skin, hair or nail that is caused by various keratinophilic fungi (dermatophytes). Dermatophytosis is a common infection throughout the world including all parts of Iran. As conventional laboratory procedures for identification ...
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Background and aim: Dermatophytosis (tinea, ringworm) is the infection of skin, hair or nail that is caused by various keratinophilic fungi (dermatophytes). Dermatophytosis is a common infection throughout the world including all parts of Iran. As conventional laboratory procedures for identification of different dermatophytes are slow or lack specificity, more rapid and reliable methods are still required.Materials and methods: Dermatophyte fungi were isolated from patients with dermatophytosis and preliminarily identified by macroscopic and microscopic morphological criteria. Total cellular DNA was extracted from isolates using conical grinder. ITS1-5.8s-ITS2 region of rDNA region was amplified by the universal fungal primers ITS1 and ITS4 and digested with Eco RII enzyme.Results: 650-750 bp band was produced , as expected. Digestion of the PCR products with the restriction enzyme EcoRII produced different electrophoretic pattern and allowed us the identification and differentiation of common pathogenic dermatophytes including Trichophyton rubrum, T. interdigital, T. mentagrophytes, T. tonsurans, T.violaceum, T. schoenleinii, T. verrucosum, M.canis, M.gypseum and Epidermophyton floccosum. Conclusion: It seems that this PCR-restriction enzyme (PCR-RE) profile is a rapid and reliable tool for discrimination of important dermatophytes and can be an applicable method in reference medical mycology laboratories for diagnostic, as well as for large-scale epidemiological purposes.